Purity: > 90 % by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT . Estradiol 17-beta-dehydrogenase 1, also known as HSD17B1, belongs to the short-chain dehydrogenases/reductases (SDR) family. This protein favors the reduction of estrogens and androgens. Recombinant human HSD17B1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.