The ALPHA CrossLaps? (CTX-I) EIA is based on one highly specific monoclonal antibodies against the amino acid sequence of EKAHDGGR. In order to obtain a specific signal in the ALPHA CrossLaps? (CTX-I) EIA , two chains of EKAHDGGR must be cross linked. Standards, control, or unknown urine samples are pipetted into the appropriate microtitre wells coated with streptavidin, followed by application of a mixture of biotinylated antibody and peroxidase-conjugated antibody. Then, a complex between ALPHA CrossLaps antigens, biotinylated antibody and peroxidase-conjugated antibody is generated, and this complex binds to the streptavidin surface via the biotinylated antibody. Following the one step incubation at 2-8°C, the wells are emptied and washed. A chromogenic substrate is added and the colour reaction is stopped with sulfuric acid. Finally, the absorbance is measured.
Size
96 wells
Concentration
n/a
Applications
colorimetric ELISA or EIA
Other Names
crosslaps, non-isomerized fragments of C-terminal telopeptides of Type-I collagen (α CTX-I) kit, assay, immunoassay