The human C1s ELISA Pair Set is for the quantitative determination of human C1s.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for C1s coatedon a 96-well plate. Standards and samples are added to the wells, and any C1spresent binds to the immobilized antibody. The wells are washed and ahorseradish peroxidase conjugated mouse anti-C1s monoclonal antibody is thenadded, producing an antibody-antigen-antibody sandwich. The wells are againwashed and TMB substrate solution is loaded, which produces color in proportionto the amount of C1s present in the sample. To end the enzyme reaction, thestop solution is added and absorbances of the microwell are read at 450 nm.