The microplate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid P Component (SAP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Serum Amyloid P Component (SAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity