Recombinant human Rb (48 - 378) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
Size
20 μg
Concentration
0.2 μg/μL
Applications
Western Blot. Rb Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. Rb Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~62kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.