Recombinant full-length human SMAD4 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.
Size
20 μg
Concentration
0.2 μg/μL
Applications
Western Blot. SMAD4 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. SMAD4 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~95kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Other Names
JIP, DPC4, MADH4, Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4