Recombinant full-length human SMAD5 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
Size
50 μg
Concentration
0.2 μg/μL
Applications
Western Blot. SMAD5 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. SMAD5 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~82kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Other Names
Dwfc, JV5-1, MADH5, DKFZp781C1895, DKFZp781O1323, Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5