GST, 1-218aa, Schistosoma japonicum, His tag, E.coli (Bioactivity Validated)
Description
Purity: > 95% by SDS - PAGE Format: Liquid. In Phosphate buffered saline (pH7.4), 10% glycerol. Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E.coli source. Recombinant Schistosoma japonicum GST, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.