Purity > 90% as determined by SDS-PAGE. MW: 35.3kDa. Full Length of Mature Protein: 27-319aa. N-terminal 6xHis-tagged. Staphylococcus aureus (strain NCTC 8325). Alpha-toxin binds to the mbrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.