Recombinant Human Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (ISCU)
Description
Purity > 90% as determined by SDS-PAGE. MW: 45.0kDa. Full Length: 1-167aa. N-terminal GST-tagged. Homo sapiens (Human). Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH
Size
100ug
Concentration
n/a
Applications
RUO
Other Names
NifU-like N-terminal domain-containing protein; NifU-like protein