MW: 12500. Purity >95% by SDS-PAGE analysis. To begin the purification process, CTI is extracted from the kernels of fresh sweet corn into a physiologic buffer. The extract is then de-fatted using acetone, and the protein is further purified by employing gel filtration and ion-exchange chromatography. The final CTI preparation appears as a single band by SDS-PAGE analyses under both reducing and non-reducing conditions. Preparations of CTI are tested for the ability to prolong the aPTT assay wit