Netrin-1, Recombinant, Human, Fc Chimera (NTN1, NTN1L, Netrin 1)
Description
Purity ~95% (SDS-PAGE). Human Netrin-1 (netr: Sanskrit for ``one who guides'') is a 75kD glycoprotein that is closely related to the laminin y domain and functions as a chemoattractive or chemorepulsive guidance cue in the central nervous system (CNS) during development (1-2). The protein is synthesized as a 604aa precursor that contains a 24aa signal sequence and a 580aa mature chain. Residues 46-283 constitute a laminin N-terminal domain (domain VI), while 285-453 make up three laminin-type epidermal growth factor-like domains. There is a final domain that runs from aa487-601 that qualifies as a Netrin- 1 like domain. It is also known as domain C in the context of C. elegans. There are four potential sites for N-linked glycosylation. Human Netrin-1 is 99% aa identical to mouse and rat Netrin-1. Netrin-1 is expressed in adult and embryonic tissues. In the adult, the protein is expressed in Schwann cells, oligodendrocytes and multiple neurons. In embryonic tissues, Netrin-1 is found in s