20-700. Mature full length protein. MW: 82.2kD. Purity >90% as determined by SDS-PAGE. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'.
Molecular cloning of the cDNA for the large subunit of the high-Ca2 -requiring form of human Ca2 -activated neutral protease.Imajoh S., Aoki K., Ohno S., Emori Y., Kawasaki H., Sugihara H., Suzuki K.Biochemistry 27:8122-8128(1988) cDNA cloning by amplification of circularized first strand cDNAs reveals non-IRE-regulated iron-responsive mRNAs.Ye Z., Connor J.R.Biochem. Biophys. Res. Commun. 275:223-227(2000) Complete sequencing and characterization of 21,243 full-length human cDNAs.Ota T., Suzuki