Purity: > 85% by SDS - PAGE. Format: Liquid. In phosphate buffered saline (pH7.4) containing 10% glycerol.. CD74, also known as HLA class II histocompatibility antigen gamma chain or HLA-DR antigens-associated invariant chain or CD74 (Cluster of Differentiation 74). The nascent MHC class II protein in the rough ER has its peptide-binding cleft blocked by the invariant chain (Ii; a trimer) to prevent it from binding cellular peptides or peptides from the endogenous pathway. The invariant chain also facilitates MHC class II's export from the ER in a vesicle. The signal for endosomal targeting resides in the cytoplasmic tail of the invariant chain. This fuses with a lateendosome containing the endocytosed, degraded proteins. It is then cleaved by cathepsin S (cathepsin L in cortical thymic epithelial cells), leaving only a small fragment calle CLIP which blocks peptide binding until HLA-DM binds to MHC II, releasing CLIP and allowing other peptides to bind. The stable MHC class-II is then presented on the cell surface. Recombinant human CD74, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Size
250ug, 50ug, 10ug
Concentration
n/a
Applications
SDS-PAGE
Other Names
HLA class II histocompatibility antigen gamma chain isoform b, DHLAG, HLADG, Ia-GAMMA II