Complex IV (cytochrome c oxidase) is the terminal electron acceptor in the mitochondrial electron transport chain. Complex IV functions by oxidizing cytochrome c and completing a four-electron reduction of oxygen (O2) to form water. During this process, two H+ are translocated from the mitochondrial matrix to the intermembrane space, contributing the mitochondrial membrane potential required for ATP synthesis. Common inhibitors of complex IV include potassium cyanide, azide, O2 limitation, nitric oxide, and carbon monoxide. Cayman's MitoCheck? Complex IV Activity Assay is designed to measure the direct oxidation of cytochrome c by complex IV, in an isolated bovine heart mitochondrial system that is supplied within the kit.
Size
96 well
Concentration
n/a
Applications
n/a
Other Names
kits assays measures measurements complexes mitochondrias mitochondrions 4 four cytochromes C oxidases terminals terminus e- electrons acceptors mitochondrials transports chains ETCs functions oxidizes oxidizing reductions redox reactions oxygens O2 water H2O 2 H+ two protons translocated translocations matices matrix intermembranes spaces membranes potentials ATP adenosines triphosphates synthesis syntheses directs oxidations isolates isolated bovines cows hearts systems inhibits inhibitors inhibitions potassiums cyanides KCN azides limits limitations nitric oxides NOs carbons monoxides COs