The microtiter plate of the Calretinin ELISA is coated with a capture antibody. The diluted sample is added, and any antigen present binds to capture antibody. After a washing step the detecting antibody (biotinylated anti-calretinin antibody) is added and binds to antigen. After another washing step the enzyme conjugate streptavidin-peroxidase is added and binds to detecting antibody. The following substrate TMB / peroxidase reaction is monitored at 450 nm (reference wavelength at 620 nm). Sample Types: EDTA plasma, serum. Range: 0.25- 4.0 ng/mL. Sensitivity: 0.05 ng/mL. Time: 4.5 hours ? Two Million tons of asbestos are still being manufactured annually. Over 100,000 people die each year from cancer diseases caused by exposure to asbestos. The most common types of asbestos-related cancer diseases are malignant lung tumors and pleural mesothelioma. Asbestos-associated cancers remain on a high level. Blood-based markers are urgently needed to limit invasive diagnostic procedures. Calretinin levels are significantly increased in malignant mesothelioma according to recent studies. The Institute for Prevention and Occupational Medicine of the German Social Accident Insurance (IPA) and DLD Diagnostika have developed a new Sandwich ELISA for the early detection of human calretinin and is distributed by Eagle Biosciences.