Llama IgG has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. This product contains equal amounts of IgG1, IgG2 and IgG3. Coomassie stained SDS-PAGE of non-reduced llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain); non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa; non-reduced llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa.
Size
0.5 mg
Concentration
1.0 mg/ml
Applications
ELISA, Flow Cytometry, Western Blot. Llama IgG whole molecule can be utilized as a control or standard reagent in SDS, Western Blotting, flow cytometry, and ELISA experiments. Specific conditions for reactivity should be optimized by the end user.Llama IgG whole molecule buffered in 0.015 M Potassium Phosphate, 0.01125 M Sodium Chloride, pH 7.2 and 25% glycerol.
Other Names
Immunoglobulin G from Llama, IgG from Llama, Llama IgG, Llama IgG Whole Molecule