pVisionGFP-N1 vector is a mammalian expression vector encoding green fluorescent protein, VisionGFP. pVisionGFP-N vector is designed to generate fusions to VisionGFP N-terminus for expression, localization and cellular dynamics studies or to express VisionGFP in eukaryotic (mammalian) cells. pVisionGFP-N vector carries synthetic version of the VisionGFP gene which codon usage is humanized, i.e. optimized for high expression in mammalian cells. pVisionGFP-N vector backbone contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication in mammalian cells, pUC origin of replication for propagation in E. coli, and f1 origin for single-stranded DNA production. SV40 early promoter provides neomy