human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail
Isotype
n/a
Format
immunoaffinity purified
Size
100 μg
Concentration
1.0 mg/mL
Applications
ELISA, Immunohistochemistry, Western Blot. This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.
Reviews / Ratings
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Description
This affinity-purified antibody is directed against human Gli-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail based on 100% sequence homology with the immunogen. Reactivity is also expected against homologues from mouse (94%) and rat (88%) based on partial homology. Reactivity with Gli-3 from other sources is not known.
Immunogen
This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to amino acids 41-57 of human Gli-3 protein.
Other Names
Rabbit anti-GLI-3 antibody, Transcriptional activator GLI3, Gli 3, GLI3 form of 190 kDa, GLI3 form of 83 kDa