Mouse Cochlear Endothelial Cells are isolated and purified by flow cytometry based on isolectin B4 sorting. Sorted cells were validated with anti-von vWF antibody in immunohistochemical staining. The cochlear microvascular endothelial cells, along with perivascular resident macrophate-type melanocytes (PVM/Ms) and cochlear pericytes, are important for maintaining the inner ear homeostasis. The cochlear microvascular endothelial cells form tight junctions and primarily sustains the barrier integrity. The cells can be used in vascular dysfunction-related hearing loss study.