This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Leukotriene B4 (LTB4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Leukotriene B4 (LTB4) and unlabeled Leukotriene B4 (LTB4) (Standards or samples) with the pre-coated antibody specific to Leukotriene B4 (LTB4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is ad