Adenosine, a purine nucleoside, is present throughout the body. It plays an important role in energy transfer via the formation of ATP, ADP and AMP and in signal transduction via the formation of cAMP. Adenosine mediates its effects directly via adenosine receptors A1, A2A, A2B and A3. It regulates myocardial oxygen consumption and coronary blood flow, exerts anti-inflammatory effects throughout the body and also regulates the Renin-Angiotensin system. It also plays a role in tissue damage and repair, and cell death. Plasma adenosine levels are increased in patients with ischemic and non-ischemic heart failure. In BioVision's Adenosine Assay, adenosine is measured using adenosine deaminase followed by a multi-step enzymatic approach resulti