Leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. Originally discovered in the mouse, the cDNA for leptin has been so far cloned from a variety of species including human, rat, cow, chicken, pig, sheep and dog. Recombinant mouse leptin was overexpressed as insoluble protein aggregate in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Alternative Names: Obese protein protein, Obesity factor protein, LEP protein, OB protein, , Leptin Mouse protein, Leptin protein, OBS protein