Recombinant Mouse DNA-dependent protein kinase catalytic subunit
Description
3747-4015aa; Partial. MW: 34.7kD. Purity >90% as determined by SDS-PAGE. Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artis (DCLRE1C). The assembly of the DNA-PK complex at DN
Nonsense mutation at Tyr-4046 in the DNA-dependent protein kinase catalytic subunit of severe combined immune deficiency mice.Araki R., Fujimori A., Hamatani K., Mita K., Saito T., Mori M., Fukumura R., Morimyo M., Muto M., Itoh M., Tatsumi K., Abe M.Proc. Natl. Acad. Sci. U.S.A. 94:2438-2443(1997) Murine cell line SX9 bearing a mutation in the DNA-PKcs gene exhibits aberrant V(D)J recombination not only in the coding joint but also in the signal joint.Fukumura R., Araki R., Fujimori A., Mori M.