This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by enzyme digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Human IgM, anti-Human IgG F(ab')2 and anti-Human Serum. No reaction was observed against anti-Human IgM Fc5μ.
Size
1 mg
Concentration
1.0 mg/mL
Applications
Monovalent Fab fragments of affinity-purified, secondary antibodies are offered to cover (block) the surface of immunoglobulins for double labeling primary antibodies from the same host species, or to block endogenous immunoglobulins in tissue sections or on cell surfaces. They can be used for these purposes because each Fab fragment has only a single antigen binding site. HUMAN IgM (myeloma) Fab μ fragment Biotin conjugated can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by the user.
Other Names
Human IgM Fab μ fragment Biotin conjugated, Human myeloma IgM Fabμ fragment Biotin conjugation