This assay has high sensitivity and excellent specificity for detection of Semaphorin 5A. No significant cross-reactivity or interference between Semaphorin 5A and analogues was observed. Sample types: tissue homogenates, cell lysates and other biological fluids. Range: 0.78-50 ng/mL. Sensitivity: 0.31 ng/mL. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 5A were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 5A were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV<10%;Inter-Assay: CV<12%. Time: 3 hours