Highly Purified (~99%). Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a.2-130(end)), H2B (a.a.2-126(end)), H3 (a.a.2-137(end)), and H4 (a.a.2-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag each H2B has a C-terminal Cys added as a biotinylation site.Total histone MW (before biotinylation) =113.8kD. Source: Recombinant protein corresponding to aa2-137 of Nucleosome at the N-terminus, expressed in E.coli. Molecular Weight: ~113.8kD Application: Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.
Size
20ug
Concentration
n/a
Applications
n/a
Other Names
n/a
Gene, Accession, CAS #
Accession: NM_033445, NM_003528, NM_003532, and NM_003549