CD14+ cells are either selected or depleted by incubating your sample with the biotin conjugated anti-human CD14 antibody followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD14+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. CD14 is expressed at high levels on human monocytes and macrophages and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cell, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in pathogen clearance and upregulation of adhesion molecules and cytokine expression. Clone 63D3 has been reported not to block LPS induced signal.