In this two-step kit, monocytes are first depleted by incubating PBMCs with biotin anti-human CD14 antibody, followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator; the unlabeled fraction is collected in a clean tube. In the second step, CD4+ T cells are positively selected from the previous unlabeled fraction by incubating it with directly conjugated Human CD4 Nanobeads. The second magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD4+ T cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Size
200 tests
Concentration
n/a
Applications
Cell Sep - Pos, Cell Sep - Neg
Other Names
Human CD4 Positive Selection, CD4 Positive Selection, CD4 Enrichment, MojoSort? Human CD4 Isolation Kit, MojoSort?