Caspases are members of the aspartate-specific cysteinyl protease family that play a central role in apoptosis. Apoptosis is involved in a variety of physiological and pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Caspase-3 is key biomarker in the assessment of apoptosis and in understanding mechanism of apoptosis induction. Caspase-3 Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product. The fluorescence intensity (lexc/em=400/490nm) is proportional to the caspace-3 activity. Key Features: Safe. Non-radioactive assay. Convenient and high-throughput. Homogeneous mix-incubate-measure type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Applications: Determination of caspase-3 activity