Mesenchymal stem cells (MSC) are well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factor will differentiate into chondrocytes, whereas MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. MSC has the capability for renewal and differentiation into various lineages of mesenchymal tissues. In essence MSC could be cultured to expand their numbers then transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs. HMSC-ad from AcceGen Biotech Research Laboratories are isolated from human adipose tissue. HMSC-ad are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HMSC-ad are characterized by immunofluorescent method with antibodies to CD73, CD90, CD105 and lipid staining after differentiation. HMSC-ad are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMSC-ad are guaranteed to further culture at the conditions provided by AcceGen Biotech Research Laboratories.