Canine Tracheal and Bronchial Epithelial Cells from Creative Bioarray are isolated from tracheal and bronchial tissue of pathogen-free laboratory Canine. Canine Tracheal and Bronchial Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray's Culture Complete Growth Medium for 3-5 days. Prior to shipping, cells at passage 2 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.