An antineoplastic antiproliferative agent; induces toxicity in an ovarian cancer cell line in vitro when used at a concentration of 10 μg/ml, with radiolabeled thymidine uptake equal to 102 and 75% of controls after 48 and 120 hr, respectively; is demethylated in vivo to form bioactive metabolites, which may exert their cytotoxic effect by cross-linking to DNA or generating formaldehyde; reduces colony survival in A204 cells in an NADP-dependent manner in the presence of an S-9 hepatic activating mixture (88 and 30% colony survival without and with NADP, respectively); inhibits tumor growth in vivo of M5076 reticulum cell sarcoma cells by 70% when used at a dose of 150 mg/kg; acts as a chemosterilant in houseflies; a concentration of 0.05% in fly food prevents hatching and pupation in male and female houseflies