Glucose Assay Kit is an Assay Kit by HK Method.This product is used to determine glucose content in human serum.Hexokinase catalyzes the reaction between glucose in the human serum and ATP to generate glucose - 6 - phosphate (G - 6 - P) and ADP. The G - 6 - P is oxidized by glucose - 6 - phosphate dehydrogenase (G - 6 - PD H) and forms 6 - p hosphogluconic acid (6 - GP), at the same time, reduction re action is occurred to transform NAD+ to NADH. The content of NADH is proportional to the glucose concentration. The glucose concentration in the serum is calculated by detecting the increase value of NADH absorbance at 340nm. table, th, td { border: 1px solid black; border-collapse: collapse; } th, td { padding: 10px; } R1: Detection Reagent 1 R2: Detection Reagent 2 ATP → 0.66 g/l Hexokinase → 30 KU/l NAD+ → 0.4 g/l G-6-PDH → 35 KU/l This assay kit is s uitable for automatic or semi - automatic biochemical analyzer with 340nm and 405nm wavelength.The sample should be fasting serum. Sample s testing should be completed at the same day after collected. Otherwise, the samples should be cryopreserved and a void repeated freeze - thaw cycles. Glucose in the samples remain stable for 7 days at 2 - 8°C or for 3 months at cryopreservation condition.. Range: 0.03 mmol/l - 40.00 mmol/l.