Enzyme Immunoassay for the quantitative determination of Serotonin In serum, unne and platelets. For in-vitro diagnostic use only.In the first step, Serotonin is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen IS bound to the solid phase of the mlcrotlter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free an