Purity: > 85% by SDS - PAGE Format: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. SUMF1, also known as sulfatase-modifying factor 1 isoform 1, is a Ca2+-binging member of the sulfatase-modifying factor family. This protein as soluble ER lumenal glycoprotein converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, it can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF2. The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in inactive FGE, and subsequently multiple sulfatase deficiency, a lysosomal storage disorder. Recombinant Human SUMF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.