The microplate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein (S100). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein (S100). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the in