Purity: > 85% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 0.15M NaCl, 30% glycerol, 1mM DTT. . SPAG7 contains 1 R3H domain. This protein is detected in fetal brain and localized in the nucleus. Recombinant human SPAG7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.