HCV-NS4-G3, Mosaic (residues 1691-1710, 1712-1733, 1921-1940) protein.Recombinant HCV-NS4 contains the fragments of theNS4 immunodominant regions: amino acids 1691-1710,1712-1733, and 1921-1940.Hepatitis C Virus NS4 is purified by proprietary chromatographic techniques.The genome of hepatitis C virus (HCV) consists of sevenfunctional regions: the core, E1, E2/NS1, NS2, NS3,NS4, and NS5 regions. Since the discovery of HCV,significant progress in the development of serologic testsfor the detection of antibodies to HCV has been made.The earliest tests, developed for blood screening, wereenzyme immunoassays (EIAs) that detect antibody to acloned HCV NS4 protein (C100).The commercial EIAs that have been developed to datehave used synthetic peptides or recombinant chimericpolyproteins as antigens. Purity >95% by SDS-PAGE and RP-HPLC