BioVision's α-enolase ELISA kit is based on Sandwich ELISA method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to ENO1. Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP) - conjugated antibody specific for ENO1 is added to each Micro Elisa strip plate well and after incubation, all unbound components are washed away. The TMB substrate solution is added to each well. The wells that contain ENO1 and HRP conjugated ENO1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ENO1. The concentration of ENO1 in the samples can be calculated by comparing the OD of the samples to the standard curve.
Size
100 assays
Concentration
n/a
Applications
A Sandwich ELISA kit for the quantitative measurement of Alpha-enolase in human serum, plasma, culture media samples