This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with PSA-NCAM . During the reaction,PSA-NCAM in the sample or standard competes with a fixed amount of PSA-NCAM on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to PSA-NCAM . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate s