Sample Type: Serum, plasma and other biological fluids. Intended Use: For quantitative detection of Septin 9 in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples. Sensitivity: <0.188ng/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Range: 0.313-20ng/ml Specificity: Human Test Principle: This BioAssay? kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Septin 9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Septin 9 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic s