100 bp DNA ladder is composed of 9 bands which are 100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 600 bp, 800 bp, 1,000 bp and 1,500 bp. The 500 bp band with higher Concentration is distinguished easily from others. Quality Control Agarose gel analysis shows that the bands between 100 bp and 1,500 bp are accurate in size and distinguishable. The 500 bp band is easily identified than any other bands.Incubation of 5 μl of the 100 bp DNA Ladder in restriction.