this is a non-secreting clone of P3X63Ag8; kappa chains are synthesized but not secreted; the cells are resistant to 0.1mM 8-azaguanine and do not grow in HAT medium; the cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity; tested and found negative for ectromelia virus (mouse pox)Tumorigenecity:noIsoenzyme:kappa light chainHistopathology:myeloma; plasmacytomaSubculture:Split ratio:Media change:Reverse transcritase:Productionimmunoglobulin(not secreted)