Rat Resident Peritoneal Macrophages are derived from the peritoneal cavity of Sprague-Dawley Rats. Prior to shipping, cells are immediately cryo-preserved in vials. Each vial contains at least 9 x10^6 cells and is delivered frozen.Rat Resident Peritoneal Macrophages were tested for expression of markers using antibodies, CD11b by flow cytometry. Rat Resident Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen Biotech. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.