Infectious culture supernatant is collected and Rotavirus is purified from infected cells and the culture medium by selective precipitation. The precipitate is then pelleted by centrifugation and resuspended in buffer. Rheoviridae/Rotavirus/Rotavirus A/Simian rotavirus SA11. Virions consist of a capsid, a core, and a nucleoprotein complex. Virus capsid is not enveloped. Capsid/nucleocapsid is isometric with icosahedral symmetry and has a diameter of 80 nm. The genome is segmented and consists of eleven segments of linear double-stranded RNA. Rotavirus is the most common cause of severe diarrhea among children. The incubation period for rotavirus disease is approximately 2 days. The disease is characterized by vomiting and watery diarrhea for 3 - 8 days, and fever and abdominal pain occur frequently. Source: Rotavirus, strain Simian rotavirus SA11, from MA104 cells. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: Rotavirus antigen is inactivated using gamma irradiation. This procedure is effective primarily by damaging viral genetic material. Rotavirus inactivation is assessed using a doub