Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 20% glycerol . 6-Pyruvoyltetrahydropterin synthase (PTS), also known as PTPS, belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. Recombinant human PTS, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.