Purity: > 90% by SDS - PAGE Format: Liquid. In phosphate buffered saline (pH7.4) containing 50% glycerol, 5mM DTT, 1mM EDTA. . PYCR2 also known as pyrroline-5-carboxylate reductase 2 isoform 1, belongs to the pyrroline-5-carboxylate reductase family. This protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. The 3 substrates of this enzyme are L-proline, NAD+, and NADP+, whereas its 4 products are 1-pyrroline-5-carboxylate, NADH, NADPH, and H+. Recombinant human PYCR2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.