Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 0.2M NaCl, 2mM EDTA. Activating transcription factor 1, also known as ATF1, is a cyclic-AMP dependent transcription factor. ATF1 is expressed in a wide variety of cell types and is capable of dimerizing with CREB. MSK1 and MSK2 protein kinases are required for the stress-induced phosphorylation of transcription factors CREB and ATF1 in primary embryonic fibroblasts. Epidermal growth factor induction of c-jun expression requires ATF1 and MEF2 sites in the c-jun promoter. Recombinant human ATF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.