Purity: > 98% by SDS - PAGE Format: Liquid. In 50 mM Tris-HCl buffer (pH 8.0) containing 0.5 mM DTT 10% glycerol . The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF was expressed in E.coli and purified by conventional chromatography techniques